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Image Search Results
Journal: Scientific Reports
Article Title: CTCF contributes in a critical way to spermatogenesis and male fertility
doi: 10.1038/srep28355
Figure Lengend Snippet: ( A ) Staining of spermatocytes at different stages of meiosis in wild-type testis using DAPI (stains DNA), a SYCP3 antibody and a CTCF antibody. SYCP3 labels the axis of meiotic chromosomes in a pattern unique to the different stages of prophase I, identifying spermatocytes at the leptotene (L), zygotene (Z), pachytene (P) and diplotene (D) stages of meiosis. The CTCF antibody preferentially labels the XY body in pachytene and diplotene meiotic cells in wild-type testis. ( B ) Staining of spermatocytes at different stages of meiosis in Ctcf -cKO testis using DAPI (stains DNA), a SYCP3 antibody and a CTCF antibody. A strong reduction of CTCF levels was observed in meiotic cells in Ctcf -cKO testes. Scale bars represent 10 micrometers.
Article Snippet: The following antibodies and dilutions were used: mouse anti-SYCP3 (Santa Cruz Biotechnology), 1:400;
Techniques: Staining
Journal: Scientific Reports
Article Title: CTCF contributes in a critical way to spermatogenesis and male fertility
doi: 10.1038/srep28355
Figure Lengend Snippet: ( A,B ) Labeling of wild-type and Ctcf -cKO spermatocytes with antibodies against SYCP3, SYCE2 and γH2AX. γH2AX labels DNA double strand breaks and the XY body, whereas SYCP3 and SYCE2 label the axis of homologous chromosomes and regions of synapsis between homologous chromosomes, respectively. Leptotene (L), Zigotene (Z), Pachytene (P) and Diplotene (D). ( C,D ) (i) Normal distribution of γH2AX at the XY body. (ii) Abnormal distribution of γH2AX at the XY body and automosomic axes in pachytene (around 50%) and diplotene (around 50%) spermatocytes of Ctcf -cKO testis. Labeling of spermatocytes at the pachytene and diplotene stages of meiosis was done using antibodies against SYCP3, histone H1t and γH2AX. H1t accumulates in spermatocytes at the midpachytene to diplotene stages of meiosis. Four mice of each genotype were used. Scale bars represent 10 micrometers.
Article Snippet: The following antibodies and dilutions were used: mouse anti-SYCP3 (Santa Cruz Biotechnology), 1:400;
Techniques: Labeling